A CSTR with a volume of 100 m is operated in cell recycle mode. At a volumetric flow rate of 10 m day and effluent biomass of 20 mg L, the steady state biomass concentration is 200 mg L. The mean cell retention time in the reactor is ____ days. (answer in integer)
GATE 2026 BT Q.56: solution and answer
Answer and solution
ANSWER
The mean cell retention time is 100 days.
Official answer: 100Source: Official answer key, GATE 2026 BT (IIT Guwahati)
Symbols: = reactor volume, = volumetric flow rate, = steady-state biomass concentration inside the reactor, = biomass concentration in the effluent, = mean cell retention time (also called solids retention time).
Definition: . The question gives no separate wastage stream, so all the biomass that leaves goes out in the effluent, at flow and concentration .
Biomass held in the reactor: .
Rate at which biomass leaves: . Both concentrations are in and both volumes in , so these units cancel and only is left.
Check: the hydraulic retention time is days. Recycle keeps cells in the reactor times longer than the liquid, so days.
KEY IDEA
Mean cell retention time is the biomass held in the reactor divided by the rate at which biomass leaves it: when cells leave only in the effluent. With cell recycle, equals the hydraulic retention time multiplied by .
WATCH OUT FOR
Answering with the hydraulic retention time days, which is the mean cell retention time only when there is no recycle and . A second mistake is to divide by , using the reactor concentration in place of the effluent concentration, which also gives 10 days.
From the GATE 2026 BT paper. Every question of this paper · Download the full paper · All 24 Bioreactor operation questions, with notes
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